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KMID : 0382619900100010485
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Hanyang Journal of Medicine
1990 Volume.10 No. 1 p.485 ~ p.494
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The Effect of 5-Fluorouracil on Conjunctival Fibronectin
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송기영/Song, Ki-Yung
고면규/최준규/Ko, Myung-Kyoo/Choe, Joon-Kiu
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Abstract
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The success of the filtering in glaucoma is dependent on the patency of the outflow pathway of the aqueous humor from the anterior chamber to the filtering bleb. This is influenced by many factors such as surgical technique, physiologic and pharmacologic factors. Most of the failure of the filtering bleb formation has resulted from the scar formation of the bleb. This is mainly derived from the fibroblast proliferation which blocks the patent bleb site.
The purpose of this study is to inverstigate the effect of the 5-FU on the extracellular matrix. This involves mainly fibronectin excreted from in vitro, by means of the immunocytochemical stain, at the ultrastructural level.
The rabbit conjunctival fibroblast and blood vessel in the conjunctival stroma were excised and placed in a culture dish containing D-MEM and fetal calf serum. After 2nd week cultivation in 5% CO₂, and 95% air humidified at 37℃, the 5-FU(0.5㎎/ℓ) was added. At the 4th week, the culture was terminated for immunocytochemical electron microscopy. The tissue was stained with antifibronectin antibody, followed by incubation with biotin-conjugated goat anti-rabbit IgG. After labelled with HRP-labelled avidin, the tissue was stained with Diaminobenzidine.
The fibroblast in the control group showed an indented nucleus with prominent nucleoli, and numerous cytoplasmic inclusions within the cytoplasm. In addition, the cytoplasmic surface showed numerous villous processes. However, the plasma membrane was ruptured, and the microorganelles underwent degenerative changes in the fibroblast treated with the 5-FU. The immunoelectron microscopy revealed an absent or weak reaction product on the vascular wall plasma membrane of the fibroblast in the 5-FU treated specimen. In short, the basement membrane of the 5-FU added cultured cell revealed a reaction product that was faint when compared with the strong reaction product in the control group.
These results suggest, that 5-FU, in a cultured fibroblast and vessel of the conjunctiva. strongly suppresses the excretion of the extracellular matrix, particularly fibronectin. These results suggest, that the application of 5-FU in a filtering operation may be useful for the success of glaucoma surgery, by preventing the secretion of the extracellular matrix, particularly fibronectin.
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